Master Thesis Online
Development of an analytical workflow for metabolomics profiling of in vitro gastrointestinal model
Hansen, Maxime 
Promotor(s) :
Focant, Jean-François
Date of defense : 4-Sep-2023 • Permalink : http://hdl.handle.net/2268.2/18463
Hansen-Maxime-Mémoire-Version-dépôt.pdfDetails
| Title : | Development of an analytical workflow for metabolomics profiling of in vitro gastrointestinal model |
| Translated title : | [fr] Développement d'une méthode analytique pour le profilage métabolomique d'un modèle in vitro du système gastro-intestinal |
| Author : | Hansen, Maxime
|
| Date of defense : | 4-Sep-2023 |
| Advisor(s) : | Focant, Jean-François
|
| Committee's member(s) : | Stefanuto, Pierre-Hugues
Bertrand, Virginie
Far, Johann
|
| Language : | English |
| Number of pages : | 69 |
| Keywords : | [en] Metabolomic [en] Untargeted [en] Gas chromatography [en] Gastrointestinal [en] VOCs |
| Discipline(s) : | Physical, chemical, mathematical & earth Sciences > Chemistry |
| Target public : | Researchers Professionals of domain Student |
| Institution(s) : | Université de Liège, Liège, Belgique |
| Degree: | Master en sciences chimiques, à finalité didactique |
| Faculty: | Master thesis of the Faculté des Sciences |
Abstract
[en] This master thesis aims to describe the development of an analytical workflow of an in vitro model for gastrointestinal epithelial cells oxidized using a chemical agent, hydrogen peroxide. Three cellular lines, namely Caco2, HCT116 and HT29 were cultured and oxidized using H2O2. Media resulting from oxidation was collected and analyzed using multidimensional gas chromatography coupled to time-of-flight mass spectrometry (GCxGC-TOFMS). Data collected was analyzed for each cell line using multivariate statistics such as PCA to differentiate controls from oxidized cells. Several VOCs were identified as potential biomarkers already highlighted by other studies, namely hexanal and glutaric acid, butyl isobutyl ester.
File(s)
Document(s)
Cite this master thesis
All documents available on MatheO are protected by copyright and subject to the usual rules for fair use.
The University of Liège does not guarantee the scientific quality of these students' works or the accuracy of all the information they contain.
The University of Liège does not guarantee the scientific quality of these students' works or the accuracy of all the information they contain.